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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Deletion of HP1γ in cardiac myocytes affects H4K20me3 levels but does not impact cardiac growth

Fig. 3

Effect of HP1γ KO on heterochromatin formation. a Expression level of heterochromatin markers. Nuclear extract was prepared from purified CM at 8 week and 100–250 ng DNA was loaded per lane. Representative pictures of 5 biological independent experiments are shown here. b, c Localization of heterochromatic histone marks and HP1s. 8-week CMs from fl/fl control and KO (Cre;fl/fl) mice were isolated and stained with specific antibodies. Single channel images (zoomed in) of histone marks and HP1s are shown in upper panel in red. Cardiac troponin is in green (lower panel) and DNA is in blue (lower panel). Individual channel images are available in Additional file 6: Fig. S4. Scale bar in upper panel and lower panel indicate 10 and 50 μm, respectively

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