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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Histone H3K9 and H4 Acetylations and Transcription Facilitate the Initial CENP-AHCP−3 Deposition and De Novo Centromere Establishment in Caenorhabditis elegans Artificial Chromosomes

Fig. 2

Histone H3K9 and H4 acetylations are important for rapid acquisition of segregation ability on newly formed ACs. a Representative live-cell images of GFP::LacI (green) and mCherry::H2B (red) in equally segregating ACs in GFP::LacI-tethering strain (left) and non-segregating ACs in GFP::LacI::HDA-1-tethering strain (right). In the left, LacO ACs aligned with metaphase plates (blue rectangles, top left) in a 9 to 16-cell stage GFP::LacI-tethering embryo. The ACs then segregated with endogenous chromosomes in anaphase (blue rectangles, bottom left). In the right, the LacO AC was adjacent to the metaphase plate but did not align with the metaphase plate (yellow rectangle, top right) in a 9 to 16-cell stage GFP::LacI::HDA-1-tethering embryo. The AC did not segregate with endogenous chromosomes in anaphase and was passively passed on to one of the daughter cells in anaphase (yellow rectangle, bottom right). The time lapse between the two images was shown (m:ss). Scale bar represents 10 μm. b Quantification of AC segregation rates in GFP::LacI-, GFP::LacI::HDA-1- and GFP::LacI::HDA-1(H145A)-tethering embryos. AC segregation rates are scored as the % of cells with segregating ACs among all dividing cells containing ACs. AC segregation rates in 1 to 8-, and 9 to 16-cell stages are significantly decreased in GFP::LacI::HDA-1-tethering strain when compared to GFP::LacI- and GFP::LacI::HDA-1(H145A)-tethering strains. The number of cells (n) analyzed was indicated. ***p < 0.001 and *p < 0.05 by Chi-squared test. Black arcs show comparisons among GFP::LacI-, GFP::LacI::HDA-1- and GFP::LacI::HDA-1(H145A)-tethering strains at the same cell stage

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