Fig. 4

Additivity of the effect of multiple PTMs. Shown are the relative changes in free energy for different combinations of PTMs (∆∆G¹⁺²) compared to the sum of individual PTMs (∆∆G¹ + ∆∆G²). In rare cases (two outlier blue crosses), the change in ∆∆G¹⁺² due to a pair of PTMs can be significantly different from the sum of the changes in binding affinity for each of the PTMs separately (∆∆G¹ + ∆∆G²). The green dot identifies the PTM pair for which experimental data are available, [43], and the red star shows the corresponding predicted value