The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila

Table 2 Histone peptide-array data reveal the top post-translational modification recognized by Ibd1

Histone	Modification 1	Modification 2	Modification 3	Modification 4	Intensity average 6xHIS-Ibd1 (4 repetitions)	Intensity average 6xHIS-G9a (2 repetitions)
H3	K9ac	K14ac			0.95	0.02
H2a	K5ac	K9ac	K13ac		0.92	0.01
H4	*R3me2s*	K5ac	K8ac	K12ac	0.90	0.00
H4	K5ac	K8ac	K12ac	K16ac	0.89	0.03
H3	K9me3	K14ac			0.88	0.01
H3	S10P	K14ac			0.88	0.04
H4	K5ac	K8ac	K12ac		0.84	0.01
H3	T11P	K14ac			0.82	0.01
H2a	S1P	K5ac	K9ac	K13ac	0.82	0.01
H2a	S1P	K9ac	K13ac		0.81	0.01
H3	R2me2s	K4me2	R8me2a	K9me2	0.19	0.96
H3	R2me2a	K4me1	R8me2a	K9me2	0.18	0.94
H3	R2me2a	K4me2	R8me2a	K9me2	0.18	0.90
H3	K4ac	R8me2s	K9me1		0.21	0.84
H3	R2me2a	K4me2	R8me2a	K9me1	0.19	0.83
H3	R2me2a	K4me3	R8me2a	K9me2	0.18	0.80
H3	R2me2a	K4ac	R8me2a	K9me2	0.28	0.79
H3	R2me2a	K4me3	R8me2a	K9me1	0.19	0.79
H3	R2me2s	K4ac	R8me2a	K9me1	0.16	0.76
H3	R2me2s	K4me2	R8me2s	K9me1	0.17	0.76

The histone peptide array contains human histone modifications that resemble Tetrahymena’s histones. The intensity average columns show the top 10 histone modifications recognized by 6xHIS-Ibd1 and 6xHIS-G9a in italics. Bold italics means that the amino acid is not present in the Tetrahymena’s histone (see Additional file 4 for Raw Data)

ISSN: 1756-8935