|
Histone
|
Modification 1
|
Modification 2
|
Modification 3
|
Modification 4
|
Intensity average 6xHIS-Ibd1 (4 repetitions)
|
Intensity average 6xHIS-G9a (2 repetitions)
|
|---|
|
H3
|
K9ac
|
K14ac
| | |
0.95
|
0.02
|
|
H2a
|
K5ac
|
K9ac
|
K13ac
| |
0.92
|
0.01
|
|
H4
|
R3me2s
|
K5ac
|
K8ac
|
K12ac
|
0.90
|
0.00
|
|
H4
|
K5ac
|
K8ac
|
K12ac
|
K16ac
|
0.89
|
0.03
|
|
H3
|
K9me3
|
K14ac
| | |
0.88
|
0.01
|
|
H3
|
S10P
|
K14ac
| | |
0.88
|
0.04
|
|
H4
|
K5ac
|
K8ac
|
K12ac
| |
0.84
|
0.01
|
|
H3
|
T11P
|
K14ac
| | |
0.82
|
0.01
|
|
H2a
|
S1P
|
K5ac
|
K9ac
|
K13ac
|
0.82
|
0.01
|
|
H2a
|
S1P
|
K9ac
|
K13ac
| |
0.81
|
0.01
|
|
H3
|
R2me2s
|
K4me2
|
R8me2a
|
K9me2
|
0.19
|
0.96
|
|
H3
|
R2me2a
|
K4me1
|
R8me2a
|
K9me2
|
0.18
|
0.94
|
|
H3
|
R2me2a
|
K4me2
|
R8me2a
|
K9me2
|
0.18
|
0.90
|
|
H3
|
K4ac
|
R8me2s
|
K9me1
| |
0.21
|
0.84
|
|
H3
|
R2me2a
|
K4me2
|
R8me2a
|
K9me1
|
0.19
|
0.83
|
|
H3
|
R2me2a
|
K4me3
|
R8me2a
|
K9me2
|
0.18
|
0.80
|
|
H3
|
R2me2a
|
K4ac
|
R8me2a
|
K9me2
|
0.28
|
0.79
|
|
H3
|
R2me2a
|
K4me3
|
R8me2a
|
K9me1
|
0.19
|
0.79
|
|
H3
|
R2me2s
|
K4ac
|
R8me2a
|
K9me1
|
0.16
|
0.76
|
|
H3
|
R2me2s
|
K4me2
|
R8me2s
|
K9me1
|
0.17
|
0.76
|
- The histone peptide array contains human histone modifications that resemble Tetrahymena’s histones. The intensity average columns show the top 10 histone modifications recognized by 6xHIS-Ibd1 and 6xHIS-G9a in italics. Bold italics means that the amino acid is not present in the Tetrahymena’s histone (see Additional file 4 for Raw Data)
