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Table 2 Histone peptide-array data reveal the top post-translational modification recognized by Ibd1

From: The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila

Histone Modification 1 Modification 2 Modification 3 Modification 4 Intensity average 6xHIS-Ibd1 (4 repetitions) Intensity average 6xHIS-G9a (2 repetitions)
H3 K9ac K14ac    0.95 0.02
H2a K5ac K9ac K13ac   0.92 0.01
H4 R3me2s K5ac K8ac K12ac 0.90 0.00
H4 K5ac K8ac K12ac K16ac 0.89 0.03
H3 K9me3 K14ac    0.88 0.01
H3 S10P K14ac    0.88 0.04
H4 K5ac K8ac K12ac   0.84 0.01
H3 T11P K14ac    0.82 0.01
H2a S1P K5ac K9ac K13ac 0.82 0.01
H2a S1P K9ac K13ac   0.81 0.01
H3 R2me2s K4me2 R8me2a K9me2 0.19 0.96
H3 R2me2a K4me1 R8me2a K9me2 0.18 0.94
H3 R2me2a K4me2 R8me2a K9me2 0.18 0.90
H3 K4ac R8me2s K9me1   0.21 0.84
H3 R2me2a K4me2 R8me2a K9me1 0.19 0.83
H3 R2me2a K4me3 R8me2a K9me2 0.18 0.80
H3 R2me2a K4ac R8me2a K9me2 0.28 0.79
H3 R2me2a K4me3 R8me2a K9me1 0.19 0.79
H3 R2me2s K4ac R8me2a K9me1 0.16 0.76
H3 R2me2s K4me2 R8me2s K9me1 0.17 0.76
  1. The histone peptide array contains human histone modifications that resemble Tetrahymena’s histones. The intensity average columns show the top 10 histone modifications recognized by 6xHIS-Ibd1 and 6xHIS-G9a in italics. Bold italics means that the amino acid is not present in the Tetrahymena’s histone (see Additional file 4 for Raw Data)