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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila

Fig. 5

a Modulation of Ibd1 interactome during conjugation. Dot plot overview of the interaction partners identified with Ibd1-FZZ during vegetative growth and 5 h after initiation of conjugation. Inner circle color represents the average spectral count, the circle size maps to the relative prey abundance across all samples shown, and the circle outer edge represents the SAINT FDR, b expression analysis of IBD1-FZZ 5 h into conjugation. Whole-cell extract (WCE) and affinity purification (AP) of untagged (−, lanes 1 and 4) and tagged (+, Ibd1-FZZ, lanes 2, 3, 5 and 6) strains. In the AP samples taken during conjugation Brg1 (top panel, lanes 5 and 6) and Pdd1p cannot be detected (data not shown). Ibd1 is recognized by α-FLAG/M2 (lower panel, lanes 5 and 6)

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