Fig. 4

Ibd1 expression pattern. Ibd1-FZZ (B2086) and untagged cells after 24 h of vegetative growth (VG) and starvation (STV) and after 3, 4, 5, 6 and 8 h post-mixing (mating of Ibd1-FZZ B2086 and CU428). a Expression analysis of Ibd1-FZZ during T. thermophila’s life cycle. Whole-cell extraction followed by TCA precipitation of untagged and tagged (Ibd1-FZZ) Tetrahymena strains. Ibd1 expresses throughout the T. thermophila life cycle (bottom panel). Brg1 is a loading control for expression throughout the T. thermophila life cycle (top panel). Pdd1 is an exclusively developmental protein and is used as a control during conjugation (middle panel); b Ibd1 localizes to the MAC during growth and sexual development including meiosis. The upper row of each panel shows a cartoon of T. thermophila depicting macronucleus (MAC), micronucleus (MIC), gametic nuclei, zygotic nuclei, new MIC, anlagen (new MAC) and old mac (OM) at different stages. Untagged cells are in the second and third panels and tagged (Ibd1-FZZ) are in the 2 lower panels. DAPI localizes to nuclei and Rho α-ZZ to the tagged protein