Fig. 2

Analysis of Tetrahymena BRD-containing proteins. a Domain architecture of the identified BRD-containing proteins. Domains were predicted using the SMART web tool and Pfam domain analysis (see “Methods”), b phylogenetic analysis of Tetrahymena BRDs. The amino acid sequences of the predicted BRDs were aligned using MUSCLE. The phylogenetic analysis was carried out using the neighbor joining method with 1000 bootstrap replicas (confidence > 90% for all nodes), c predicted structure (left) of the Ibd1 BRD shown in ribbon diagram with rainbow color scheme. Blue represents the N-terminus, whereas red shows the C-terminus of the predicted structure. The superimposition (right) was carried out using the BRD of human SMARCA2 protein (PDB: 5DKC) which is shown in violet color backbone format. Note The identified Tetrahymena BRD-containing proteins were named based on the domain architecture if no clear human ortholog was available. BroW1, bromo-WD40 domain protein; BrEt, bromo-Et domain protein; BrAn, Bomo-Ank domain protein; Brop1–6, BRD-containing protein