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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Independent functions of DNMT1 and USP7 at replication foci

Fig. 3

Substitution of lysines by glutamines within (GK) repeats does not affect DNMT1 stability or function. a Positions of GK→GQ substitutions within the (GK) repeats of DNMT1. The substituted lysines correspond to those reported to be acetylated in vivo and to block the interaction of DNMT1 and USP7 in vitro. b Normal stability of GK→GQ DNMT1 in stably transfected Dnmt1-null ES cells. Two independent transfected clones are shown. c Normal methylation of genomic DNA as measured by resistance to HpaII in stably transfected ES cells at left; at center, equal methylation of IAP retrotransposon DNA by wild-type and GK→GQ DNMT1. “WT SssI” indicates DNA that had been methylated at all CpG sites by treatment with M.SssI. At right is LUMA analysis of DNA methylation in (GK) versus (GQ) DNMT1 expression cells. n = 3 (biological replicates). Error bars indicate standard deviations. The center value is mean, and p values were calculated using the two-tailed t test

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