Fig. 1

Animal breeding classification and disease. a Experimental design of F0 generation gestating female exposure and then F1, F2 and F3 generations being generated for sperm collection. The direct exposure of the F0 generation female, F1 generation fetus and F2 generation germline is also shown, b testis spermatogenic cell apoptosis as determined with TUNEL analysis with frequency (%) of apoptosis for each generation and lineage shown. Asterisk indicates statistical significance with control with a p < 0.01 with a Fisher’s exact t test