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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells

Fig. 3

TET2 targets ICRs for 5mC hydroxylation and co-regulates expression of TEs. a, b 5hmC enrichment measured by hMeDIP-qPCR in TET1 KO cells (a), TET2 KO cells (b) and their parental cell lines upon Kap1 knockdown. Error bars represent SEM, *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test. n = 4. c, d Relative expression of indicated TEs measured by RT-qPCR in TET1 KO (c) or TET2 KO (d) cells and their parental cell lines. Expression was normalized to the housekeeping gene β-actin. Error bars represent SEM, *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test. n = 4. e ChIP-qPCR for TET1 on mESCs knockdown for Kap1. FosB promoter is used as a positive control. Error bars represent SEM, *p < 0.05, **p < 0.01, ns = non-significant, Student’s t test, n = 3

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Epigenetics & Chromatin

ISSN: 1756-8935