Fig. 2

TDG localizes to sites occupied by transcription factors. a Individual datasets obtained from the Transcription Factor ChIP-Seq Uniform Peaks dataset from ENCODE were compared to the TDG dataset using the Fisher’s exact test p value and Jaccard statistic to determine relative similarity. A subset of the most similar matches is labeled with the cell type and treatment, if disclosed, in brackets. b Overlap between TDG peaks and the corresponding ENCODE dataset (The average binding profile of the 10 least similar datasets from the ENCODE database was used as controls. None of the 10 least similar had more then a single peak which overlapped with our dataset). c Motif analysis performed on sites of E2-mediated TDG localization revealed the canonical ERE as a top hit followed by GATA protein consensus binding site. d Overlap of ChIP-Seq signal from publically available histone datasets at sites of E2-dependent TDG binding in MCF7 cells. e Heatmaps showing intensity of histone marks at sites of TDG binding. Sites where TDG localizes in response to E2 are enriched for histone marks indicating “active” enhancers (H3K27Ac, H3K4me2 and H3K4me3) while depleted for those marking “repressed” enhancers (H3K9me3 and H3K27me3)