Fig. 5
From: Identification of protein lysine methylation readers with a yeast three-hybrid approach
Representative fluorescence microscopy images documenting the co-localization of new H3K9 reading candidates with CBX1-CD. Results are shown for HSFY1 (a), AGO3 (b), ZNF470 (c) and DCAF8 (d) in iMEFs WT and Suv39h1h2‒/‒ (DKO). The mVenus-tagged constructs co-localized with H3K9me3 detector mCerulean-CBX1-CD in iMEF-WT cells. The signal of mCerulean-CBX1-CD and analyzed mVenus-tagged constructs was homogenous in DKO cells. The transfection, imaging, and display settings of the images are identical. e Quantification of the biologically duplicated experiments representatively shown in a–d