Fig. 4
From: Identification of protein lysine methylation readers with a yeast three-hybrid approach
Methylation-dependent interaction of H3 (1–20) with chromodomain of CBX1 and MPP8 in yeast cells. Y2H Gold and Y187 yeast strain were transformed with bait (pBRIDGE-GAL4-BD) and prey (pGADT7-GAL4-AD) constructs, respectively. Transformed Y2H Gold cells were mated with Y187 harboring CBX1-CD, MPP8-CD in YPD-rich medium and plated in serial dilutions on selection media, either color change selection media (-Trp/-Leu/-Met/X-α-Gal) and color change and growth selection media (-Trp/-Leu/-Met/-His/X-α-Gal)