Fig. 3From: Identification of protein lysine methylation readers with a yeast three-hybrid approachExpression of bait and prey constructs. a Yeast cells transformed with bait or prey were inoculated in liquid culture and spotted in serial dilutions onto SD-Trp/-Met, SD-Trp/-Met/X-α-Gal, SD-Leu, SD-Trp/-Met/-Leu plates. b Lysates from yeast expressing GAL4-BD or -AD were analyzed by western blot using antibodies to GAL4-BD, HA-tag, and PGK as protein loading controlBack to article page