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Table 1 H2A, H2B and H4 signature peptides

From: Systematic quantitative analysis of H2A and H2B variants by targeted proteomics

Peptide N°

Protein

Peptide sequence

ESPP score

Heavy peptide identified

Light peptide identified

Identified in our discovery analyses

Recorded in PeptideAtlas

1

Canonical H2A, H2A.J, H2A.X, H2AZ.1, H2AZ.2, TS H2A.1

AGLQFPVGR

0.739

Yes

Yes

Yes

PAp00032266

2

Canonical H2A, H2A.J, TS H2A.1

VTIAQGGVLPNIQAVLLPKa

0.224

 

Yes

Yes

PAp00008634

3

H2A.X

ASQASQEY

0.278

No

No

Yes

PAp00852754

4

H2AZ.1, H2AZ.2

GDEELDSLIK

0.519

Yes

Yes

Yes

PAp00067999

5

H2AZ.1, H2AZ.2

ATIAGGGVIPHIHK

0.522

Yes

Yes

Yes

PAp00032640

6

Macro-H2A.1, Macro-H2A.2, Macro-H2A.3

AGVIFPVGR

0.575

Yes

Yes

Yes

PAp00413759

7

Macro-H2A.1, Macro-H2A.2

HILLAVANDEELNQLLK

0.222

Yes

Yes

Yes

PAp00069466

8

Macro-H2A.1

LEAIITPPPAK

0.713

Yes

Yes

Yes

PAp00429995

9

Macro-H2A.1

AASADSTTEGTPTDGFTVLSTK

0.517

Yes

Yes

No

PAp00380945

10

Macro-H2A.1

NGPLEVAGAAISAGHGLPAK

0.723

Yes

Yes

Yes

PAp00389241

11

Macro-H2A.1

SIAFPSIGSGR

0.796

Yes

Yes

Yes

PAp00077504

12

Macro-H2A.2, Macro-H2A.3

GVTIASGGVLPR

0.866

Yes

Yes

Yes

PAp00519811

13

Macro-H2A.2

SETILSPPPEK

0.580

Yes

No

Yes

 

14

Macro-H2A.2

EGTSNSTSEDGPGDGFTILSSK

0.499

No

No

Yes

 

15

Macro-H2A.2

SVAFPPFPSGR

0.578

Yes

Yes

Yes

 

16

Macro-H2A.3

NCLSAAEIR

0.726

Yes

No

No

 

17

Macro-H2A.3

SPVAETASPGRPGDPQGHLGSLR

0.664

Yes

No

No

 

18

Macro-H2A.3

AGDGQTGHQVALSGSGGEGGSA

0.504

No

No

No

 

19

TS H2A.1

QGNYAQR

0.089

Yes

No

No

 

20

H2A.L.1-H2al1a

GELPFSLVDR

0.821

Yes

Yes

Yes

 

21

H2A.L.1-H2al1a, H2al1e, H2al1k, H2al1o

IAPEDVR

0.299

No

No

  

22

H2A.L.1-H2al1b, H2al1e, H2al1k, H2al1m, H2al1n

GELPLSLVDR

0.814

Yes

Yes

Yes

 

23

H2A.L.1-H2al1b

IAPEDVHLVVQNNEQLR

0.301

Yes

No

Yes

 

24

H2A.L.1-H2al1a, H2al1e, H2al1k, H2al1m

LVVQNNEQLR

0.596

Yes

Yes

Yes

 

25

H2A.L.1-H2al1j

GEFPLSLVDR

0.821

Yes

No

  

26

H2A.L.1-H2al1j

FLPEGNHSGR

0.445

Yes

No

  

27

H2A.L.1-H2al1m

VTPEDVR

0.216

Yes

No

  

28

H2A.L.1-H2al1o

GELPLSLVDHFLR

0.262

Yes

No

  

29

H2A.L.2, Y-Chr H2A.L.3

AELQFPVSR

0.844

Yes

Yes

Yes

 

30

H2A.L.2

FLREGNYSR

0.289

No

No

  

31

H2A.L.2

IAPEHVCR

0.438

Yes

No

Yes

 

32

H2A.L.2

VVQNNEQLHQLFKa

0.322

 

Yes

Yes

 

33

Y-Chr H2A.L.3

FLGEGIYSR

0.433

Yes

No

  

34

Y-Chr H2A.L.3

IAPEHVCQVVQNK

0.464

Yes

No

  

35

H2A.P

NAPFSLFDEMPGPR

0.673

Yes

No

  

36

H2A.B.2

NTENCLQR

0.261

Yes

No

  

37

H2A.B.2, H2A.B.3

LLELAGNEAQR

0.777

Yes

No

  

38

Canonical H2B

KESYSVYVYK

0.255

Yes

Yes

Yes

PAp00035223

39

Canonical H2B

ESYSVYVYK

0.359

Yes

Yes

Yes

PAp00066832

40

Canonical H2B, TS H2B.1

EIQTAVR

0.331

Yes

Yes

Yes

PAp00066243

41

Canonical H2B, TS H2B.1

LLLPGELAK

0.517

Yes

Yes

Yes

PAp00073476

42

TS H2B.1

KESYSIYIYK

0.227

Yes

Yes

Yes

PAp00379050

43

TS H2B.1

ESYSIYIYK

0.311

Yes

Yes

Yes

 

44

subH2B

KLATLAVTFGSK

0.608

Yes

Yes

Yes

 

45

subH2B

LATLAVTFGSK

0.571

Yes

Yes

Yes

 

46

H2B.L.2

NSFAIYFPK

0.398

Yes

Yes

Yes

 

47

H2B.L.2

SVNILDSFVK

0.421

Yes

Yes

Yes

 

48

H2B.L.2

IASEASFLAR

0.728

Yes

Yes

Yes

 

49

H4

DNIQGITKPAIRa

0.845

 

Yes

Yes

PAp00033165

50

H4

RISGLIYEETRa

0.548

 

Yes

Yes

PAp00006681

51

H4

ISGLIYEETRa

0.667

 

Yes

Yes

PAp00035058

52

H4

VFLENVIRa

0.259

 

Yes

Yes

PAp00038639

53

H4

DAVTYTEHAKa

0.333

 

Yes

Yes

PAp00039819

54

H4

KTVTAMDVVYALKa

0.505

 

Yes

Yes

PAp00072198

55

H4

TVTAMDVVYALKa

0.402

_

Yes

Yes

PAp00008048

  1. The carbamidomethylated or oxidized forms of peptides containing cysteine or methionine residues, respectively, were also monitored. “Yes” indicates that peptides were successfully detected by LC-SRM or LC–MS/MS analysis and “No” indicates they failed to be
  2. aPeptides monitored only in their endogenous form