Skip to main content

Advertisement

Table 1 H2A, H2B and H4 signature peptides

From: Systematic quantitative analysis of H2A and H2B variants by targeted proteomics

Peptide N° Protein Peptide sequence ESPP score Heavy peptide identified Light peptide identified Identified in our discovery analyses Recorded in PeptideAtlas
1 Canonical H2A, H2A.J, H2A.X, H2AZ.1, H2AZ.2, TS H2A.1 AGLQFPVGR 0.739 Yes Yes Yes PAp00032266
2 Canonical H2A, H2A.J, TS H2A.1 VTIAQGGVLPNIQAVLLPKa 0.224   Yes Yes PAp00008634
3 H2A.X ASQASQEY 0.278 No No Yes PAp00852754
4 H2AZ.1, H2AZ.2 GDEELDSLIK 0.519 Yes Yes Yes PAp00067999
5 H2AZ.1, H2AZ.2 ATIAGGGVIPHIHK 0.522 Yes Yes Yes PAp00032640
6 Macro-H2A.1, Macro-H2A.2, Macro-H2A.3 AGVIFPVGR 0.575 Yes Yes Yes PAp00413759
7 Macro-H2A.1, Macro-H2A.2 HILLAVANDEELNQLLK 0.222 Yes Yes Yes PAp00069466
8 Macro-H2A.1 LEAIITPPPAK 0.713 Yes Yes Yes PAp00429995
9 Macro-H2A.1 AASADSTTEGTPTDGFTVLSTK 0.517 Yes Yes No PAp00380945
10 Macro-H2A.1 NGPLEVAGAAISAGHGLPAK 0.723 Yes Yes Yes PAp00389241
11 Macro-H2A.1 SIAFPSIGSGR 0.796 Yes Yes Yes PAp00077504
12 Macro-H2A.2, Macro-H2A.3 GVTIASGGVLPR 0.866 Yes Yes Yes PAp00519811
13 Macro-H2A.2 SETILSPPPEK 0.580 Yes No Yes  
14 Macro-H2A.2 EGTSNSTSEDGPGDGFTILSSK 0.499 No No Yes  
15 Macro-H2A.2 SVAFPPFPSGR 0.578 Yes Yes Yes  
16 Macro-H2A.3 NCLSAAEIR 0.726 Yes No No  
17 Macro-H2A.3 SPVAETASPGRPGDPQGHLGSLR 0.664 Yes No No  
18 Macro-H2A.3 AGDGQTGHQVALSGSGGEGGSA 0.504 No No No  
19 TS H2A.1 QGNYAQR 0.089 Yes No No  
20 H2A.L.1-H2al1a GELPFSLVDR 0.821 Yes Yes Yes  
21 H2A.L.1-H2al1a, H2al1e, H2al1k, H2al1o IAPEDVR 0.299 No No   
22 H2A.L.1-H2al1b, H2al1e, H2al1k, H2al1m, H2al1n GELPLSLVDR 0.814 Yes Yes Yes  
23 H2A.L.1-H2al1b IAPEDVHLVVQNNEQLR 0.301 Yes No Yes  
24 H2A.L.1-H2al1a, H2al1e, H2al1k, H2al1m LVVQNNEQLR 0.596 Yes Yes Yes  
25 H2A.L.1-H2al1j GEFPLSLVDR 0.821 Yes No   
26 H2A.L.1-H2al1j FLPEGNHSGR 0.445 Yes No   
27 H2A.L.1-H2al1m VTPEDVR 0.216 Yes No   
28 H2A.L.1-H2al1o GELPLSLVDHFLR 0.262 Yes No   
29 H2A.L.2, Y-Chr H2A.L.3 AELQFPVSR 0.844 Yes Yes Yes  
30 H2A.L.2 FLREGNYSR 0.289 No No   
31 H2A.L.2 IAPEHVCR 0.438 Yes No Yes  
32 H2A.L.2 VVQNNEQLHQLFKa 0.322   Yes Yes  
33 Y-Chr H2A.L.3 FLGEGIYSR 0.433 Yes No   
34 Y-Chr H2A.L.3 IAPEHVCQVVQNK 0.464 Yes No   
35 H2A.P NAPFSLFDEMPGPR 0.673 Yes No   
36 H2A.B.2 NTENCLQR 0.261 Yes No   
37 H2A.B.2, H2A.B.3 LLELAGNEAQR 0.777 Yes No   
38 Canonical H2B KESYSVYVYK 0.255 Yes Yes Yes PAp00035223
39 Canonical H2B ESYSVYVYK 0.359 Yes Yes Yes PAp00066832
40 Canonical H2B, TS H2B.1 EIQTAVR 0.331 Yes Yes Yes PAp00066243
41 Canonical H2B, TS H2B.1 LLLPGELAK 0.517 Yes Yes Yes PAp00073476
42 TS H2B.1 KESYSIYIYK 0.227 Yes Yes Yes PAp00379050
43 TS H2B.1 ESYSIYIYK 0.311 Yes Yes Yes  
44 subH2B KLATLAVTFGSK 0.608 Yes Yes Yes  
45 subH2B LATLAVTFGSK 0.571 Yes Yes Yes  
46 H2B.L.2 NSFAIYFPK 0.398 Yes Yes Yes  
47 H2B.L.2 SVNILDSFVK 0.421 Yes Yes Yes  
48 H2B.L.2 IASEASFLAR 0.728 Yes Yes Yes  
49 H4 DNIQGITKPAIRa 0.845   Yes Yes PAp00033165
50 H4 RISGLIYEETRa 0.548   Yes Yes PAp00006681
51 H4 ISGLIYEETRa 0.667   Yes Yes PAp00035058
52 H4 VFLENVIRa 0.259   Yes Yes PAp00038639
53 H4 DAVTYTEHAKa 0.333   Yes Yes PAp00039819
54 H4 KTVTAMDVVYALKa 0.505   Yes Yes PAp00072198
55 H4 TVTAMDVVYALKa 0.402 _ Yes Yes PAp00008048
  1. The carbamidomethylated or oxidized forms of peptides containing cysteine or methionine residues, respectively, were also monitored. “Yes” indicates that peptides were successfully detected by LC-SRM or LC–MS/MS analysis and “No” indicates they failed to be
  2. aPeptides monitored only in their endogenous form