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Fig. 7 | Epigenetics & Chromatin

Fig. 7

From: Systematic quantitative analysis of H2A and H2B variants by targeted proteomics

Fig. 7

Quantification of the abundance of H2A.L.1 isoforms in Sly-KD mice. The relative abundance of H2A and H2B variants was quantified by PRM in round spermatids from WT and Sly-KD mice. Two independent biological replicates are presented (top and bottom panels). H2A.L.2 and Y-ChrH2A.L.2 are expected to be expressed at similar levels between Sly-KD and WT spermatids [64] and peptide P29, shared by both variants, was used to normalize the abundance of peptides specific to H2A.L and subH2B (H2B.L.1) variants. The abundance of the other histone variants was normalized to H4 (P50–52). For more details, please refer to the “Methods” section

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