Fig. 6
From: Systematic quantitative analysis of H2A and H2B variants by targeted proteomics
SRM-based quantification of H2A and H2B variants during mouse spermatogenesis a Experimental design. Spermatogenic cell fractions were analyzed [meiotic spermatocytes (Sc); round spermatids (R); elongating and condensing spermatids (EC)]. Histones were extracted with sulfuric acid, digested by trypsin and analyzed by LC-SRM. b LC-SRM quantification of the abundance of histone variants during spermatogenesis. Data were normalized to H4 levels as described in the “Methods” section and in Additional file 3. Two independent biological replicates are presented for each protein (replicate 1 and replicate 2) and were analyzed in technical triplicates. c Heat maps representing the abundance of H2A and H2B variants during spermatogenesis