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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Systematic quantitative analysis of H2A and H2B variants by targeted proteomics

Fig. 5

Evaluation of the linearity of SRM quantification. a The SRM quantification is linear, using an increasing quantity of each isotopically labeled signature peptide spiked in a constant amount of protein matrix (acid-extracted histones from mouse testis). Please refer to the “Methods” section for experimental details. Data were normalized as described in Ref. [49]. b Similar results were obtained when a constant quantity of isotopically labeled signature peptide was spiked in an increasing amount of protein matrix (acid-extracted histones from mouse testis)

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