Fig. 3

Effects of treatment with 5-aza-dC on the resulting sex ratio at 90 dpf. a Sex ratio of fish treated with 5-aza-dC at 75 µM from 0 to 2 dpf. Each datapoint is the mean ± s.e.m., corresponding to five independent experiments using five different breeding pairs. Numbers of fish are 162 and 81 in control and 5-aza-dC, respectively. Significant differences among groups (P < 0.05) were analysed by the Chi-squared test with Yate’s correction. b Sex ratio of zebrafish larvae (0–6 dpf) treated with different 5-aza-dC doses (0, 5, 15, 25 µM n = 13, 27, 31, 19, respectively). Each datapoint is the mean ± s.e.m. of two independent experiments with two technical replicates each. c Sex ratio of zebrafish treated with 25 µM of 5-aza-dC during different periods during gonadal development. Data shown as mean ± s.e.m. of three independent experiments with 1–3 biological replicates (n = 133, 59, 72 and 11 in control, 10–20 dpf, 20–30 dpf and 10–30 dpf groups, respectively). Significant differences among groups were analysed by the Chi-squared test with Yate’s correction. Significant differences (P < 0.05) are indicated by different letters. N/A, variation not assessed because there was only one experiment one technical replicate