Fig. 1

Zebrafish treated with 5-aza-dC. a Survival, b hatching rate and c teratology of zebrafish embryos treated with 5-aza-dC at 75 µM from 0 to 2 dpf. Each shown datapoint is the mean ± s.e.m. of seven independent experiments. Within each experiment, each datapoint is the mean of three technical replicates. Significant differences P < 0.05 (in a) or P < 0.01 (in c) among groups at a given age are indicated by different letters and were examined by Student’s t test. d Survival of treated zebrafish with 25 µM of 5-aza-dC during gonadal development. Each datapoint is the mean ± s.e.m. of two independent experiments. Within each experiment, each datapoint is the mean of 2–4 technical replicates, originated from five breeding pairs. Significant differences (P < 0.05) among groups at a given sampling age were tested by one-way ANOVA and are indicated by different letters