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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus

Fig. 2

Co-localisation of Pleiohomeotic (Pho) and RNA polymerase II at the heat shock loci upon activation is independent of transcriptional elongation. ad Double polytene immunostaining for RNA polymerase II (green) and Pho (magenta) under control, HS (heat shock), FP (flavopiridol) control and FP HS (flavopiridol + heat shock), respectively. The white block arrows point at the double heat shock puffs (87A/C) that were observed under HS and FP HS conditions. Magnification used 63×. e, f ChIP-qPCR measurements of occupancy levels for HSF and Ph, respectively, at the hsp70 locus in S2 DRSC cells. Distances of the location of the primers used from the hsp70 TSS are as follows: for e (− 85 bp) and for f (+ 78 bp) has been depicted in the form of a cartoon below the data figure. The control bar represents cells that were maintained at 25 °C for 15 min, the HS bar represents cells that were maintained at 37 °C for 15 min, the FP control bar represents cells that were treated with 500 nM flavopiridol for 40 min and then maintained at 25 °C for 15 min and the FP HS bar represents cells that were treated with 500 nM flavopiridol for 40 min and then maintained at 37 °C for 15 min. Data information: In e, f, data are presented as mean ± SEM for n = 2

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