Fig. 7

UCHL1 and RAP1 are associated with the nuclear scaffold. a Proteins (5 µg) from each fraction [total cellular protein (TC), total nuclear protein (TN), ammonium sulfate fraction (AS) and nuclear scaffold protein (NS)] were resolved on a SDS 15% polyacrylamide gel and stained with Coomassie Blue. The DU 145 cells were treated or not with protein–protein cross-linker DSP. b Immunoblot analysis using the indicated antibodies on TC, TN, AS and NS proteins from DU 145 cells treated or not with protein–protein cross-linker DSP. PC-3 cells were not treated with DSP. c Immunoblot analysis of UCHL1 and RAP1 in nuclear scaffold protein complexes from DSP-treated DU 145 cells. The nuclear scaffold was solubilized in SDS sample buffer in both reducing (R) and non-reducing (NR) conditions and analyzed by SDS-PAGE on a 10% gels and immunoblotting