Skip to main content
Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Ubiquitin C-terminal hydrolase isozyme L1 is associated with shelterin complex at interstitial telomeric sites

Fig. 6

UCHL1 interacts with TRF2 and co-localizes with telomeres. a Double immunofluorescence displayed co-localization of TRF2 and UCHL1 in HEK293T cells. Blue—nucleus; red—UCHL1; green—TRF2; yellow—TRF2–UCHL1 co-localizing spots. b Telomeric localization of UCHL1 was determined in HEK293T cells by immuno-FISH with antibodies against UCHL1 and fluorochrome-coupled (Cy3) telomere PNA probes. c Telomeric localization of TRF2 was determined in HEK293T cells by immuno-FISH for antibodies against TRF2 and fluorochrome-coupled (Cy3) telomere PNA probes. Blue—nucleus; red—telomeres; green—UCHL1/TRF2; yellow—telomere-UCHL1/TRF2 co-localizing spots. d Thirty HEK cell nuclei per experiment were quantified. Average number of colocalized spots per nucleus was counted and graphed. Image J software was used for quantification. Error bar represents the standard error of mean

Back to article page