Fig. 6
From: Chromatin organization changes during the establishment and maintenance of the postmitotic state
Robust G0 restores heterochromatin clustering and shares features with senescence. E2F was expressed in the posterior wing to delay cell cycle exit. At 42–44 h, when cells are in robust G0, wings were dissected and immunostained for the indicated histone modifications or chromatin binding proteins and DAPI to label nuclei (A–F). The distribution of staining intensity in 509–1185 nuclei, binned into three ranges, is shown at right. Robust G0 in the presence of high E2F increases H3K27Me3, HP1 and pH2Av, chromatin marks associated with senescence. P values were determined by an unpaired t test; ****< 0.0001; ***< 0.001. Scale bars = 10 μm in A–F except for anterior (A) and posterior (P) zoomed images where the bar = 2 μm (e.g., BA, BP)