Fig. 5From: Chromatin organization changes during the establishment and maintenance of the postmitotic stateSpecific histone modifications associated with gene activation are increased when flexible G0 is compromised. E2F was expressed in the posterior wing to delay cell cycle exit. At 26–28 h wings were dissected and immunostained for the indicated histone modifications and DAPI to label nuclei (A–J). The anterior–posterior boundary is indicated by a white line. The distribution of staining intensity in 217–1312 nuclei, binned into three ranges, is shown at right. Compromising flexible G0 specifically increases H3K27ac and H4K16ac. P values were determined by an unpaired t test; ****< 0.0001. Scale bars = 10 μm in A–J except for anterior (A) and posterior (P) zoomed images where the bar = 2 μm (e.g., BA, BP)Back to article page