Fig. 3
From: Chromatin organization changes during the establishment and maintenance of the postmitotic state
Heterochromatin clustering is disrupted when G0 is compromised. E2F was co-expressed with GFP in the posterior wing (boundary indicated by a white line) from the start of metamorphosis (0 h APF) to delay cell cycle exit. At 26–28 h wings were dissected and immunostained for the indicated chromatin modifications and DAPI to label nuclei (A–I). J–M Fluorescence signal were measured for 485–848 nuclei for each chromatin modification. The distribution of overall fluorescence intensity (J), foci number per nucleus section (K) and individual focus intensity (L, M) all indicate that delaying cell cycle exit disrupts heterochromatin clustering in wing cell nuclei. Scale bars = 10 μm in A–I except for anterior (A) and posterior (P) zoomed images where the bar = 2 μm (e.g., BA, BP). P values were determined by an unpaired t test. *< 0.05; **< 0.01; ***< 0.001; ****< 0.0001