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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Chromatin organization changes during the establishment and maintenance of the postmitotic state

Fig. 1

Heterochromatin clustering increases as the cell cycle slows and cells differentiate. A Wings of the indicated developmental stages were immunostained for the indicated chromatin modifications and chromatin binding proteins. B As the cell cycle slows down and cells differentiate, the distribution of heterochromatin-associated foci shifts toward larger, brighter foci indicating increased clustering. Coalescence is quantified as the total intensity of individual focus within 129–448 nuclei at each developmental stage. C, D Kc cells treated with dsRNA against GFP, wee/myt and cycB were immunostained for the indicated chromatin modifications and fluorescence intensity was quantified. E, F Fluorescent in situ hybridization (FISH) against the rDNA ITS region was performed on wings of the indicated stages. rDNA foci coalesce and condense in postmitotic cells. G, H FISH against the AACAC pericentromeric satellite repeats was performed on wings of the indicated stages and the distance to the center of the DAPI-bright chromocenter was measured. The distance decreases in postmitotic cells indicating increased condensation of heterochromatin. I A box plot of the RNA log2-fold changes compared to proliferative L3 for each time point is shown. J A line plot of average FAIREseq signal across all accessible chromatin for the indicated stages is shown. The accessibility of regulatory elements is similar in cycling and postmitotic wings. Scale bars = 2 μm. P values were determined by an unpaired t test *< 0.05; **< 0.01; ***< 0.001; ****< 0.0001

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