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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: H2A O-GlcNAcylation at serine 40 functions genomic protection in association with acetylated H2AZ or γH2AX

Fig. 3

H2AS40Gc in sites of DNA damages. ah Level of H2AS40Gc, γH2AX, and AcH2AZ around the damaged DNA site induced by the CRISPR/CAS9 system. gRNA-target regions were selected at gene-body (chromosome 3) (a) and intergenic region (chromosome 13) (e). Level of H2AS40Gc and γH2AX at − 0.5 kb from damaged DNA site on chromosome 3 (b) and 0.5 kb from the damaged DNA site on chromosome 13 (f). ChIP signals were calculated as ChIP DNA/Input DNA and expressed as fold enrichment relative to the uncut (mESCs expressing guide RNA and nuclease-deficient CAS9). Values indicate mean ± SD (n = 3). Enrichment of H2AS40Gc and γH2AX around the DNA damage site on chromosome 3 (c, d) and chromosome 13 (g, h) induced using CRISPR/CAS9, at 16 and 24 h after transfection. Values indicate mean ± SD (n = 3). DSB, double-strand DNA break

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