Fig. 3From: H2A O-GlcNAcylation at serine 40 functions genomic protection in association with acetylated H2AZ or γH2AXH2AS40Gc in sites of DNA damages. a–h Level of H2AS40Gc, γH2AX, and AcH2AZ around the damaged DNA site induced by the CRISPR/CAS9 system. gRNA-target regions were selected at gene-body (chromosome 3) (a) and intergenic region (chromosome 13) (e). Level of H2AS40Gc and γH2AX at − 0.5 kb from damaged DNA site on chromosome 3 (b) and 0.5 kb from the damaged DNA site on chromosome 13 (f). ChIP signals were calculated as ChIP DNA/Input DNA and expressed as fold enrichment relative to the uncut (mESCs expressing guide RNA and nuclease-deficient CAS9). Values indicate mean ± SD (n = 3). Enrichment of H2AS40Gc and γH2AX around the DNA damage site on chromosome 3 (c, d) and chromosome 13 (g, h) induced using CRISPR/CAS9, at 16 and 24 h after transfection. Values indicate mean ± SD (n = 3). DSB, double-strand DNA breakBack to article page