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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: H2A O-GlcNAcylation at serine 40 functions genomic protection in association with acetylated H2AZ or γH2AX

Fig. 2

H2AS40Gc is responsive to DNA damages. ae Immunofluorescence (IF) images of H2AS40Gc, γH2AX, and AcH2AZ in mESCs treated with camptothecin (CPT) and etoposide (ETP). The lower right box in the photo is the zoomed white box in the nucleus. Bars = 5 μm. Measurement of the number of H2AS40Gc foci (b) and co-localization of H2AS40Gc with γH2AX (c) or AcH2AZ (e) in nuclei of mESCs treated with CPT and ETP. **p < 0.01. f, g Time course of accumulation of H2AS40Gc, γH2AX, and AcH2AZ proteins in ETP-treated mESCs. The graph was plotted from band intensities of f calculated by the ImageJ software (g). Values of H2AS40Gc, γH2AX, and AcH2AZ were normalized to the intensity of α-H2A, -H2AX, and -H2AZ, respectively, and expressed as a ratio relative to the maximum

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