Fig. 3

Increase in H4 acetylation associated with adaptation of monosomic Ch5 state requires an intact NuA4 complex. a Comparison of total H4 acetylation in parental C. albicans strains and monosomic Ch5 strains derived as described in the tex. Shown is total H4 acetylation in: (i) the parental strain (3153A) and the monosomic Ch5 mutant Sor125(55); (ii) the parental strain DAY286 and the monosomic Ch5 mutant DAY286-1; (iii) parental strain Nbn1 (nbn −/−) and the monosomic Ch5 mutant Nbn1-1; and (iv) the parental stran Eaf3 (eaf −/−) and the monosomic Ch5 mutant Eaf3-1. b Total H4 acetylation in deletion mutants Nbn1 and Eaf3 compared to their parental strain DAY286. H4 acetylation was determined by Western blot with the indicated antibodies and results normalized to the parental strain response for each set (see text). Data are averaged from three independent experiments. Asterisks indicate statistical significance of the difference in acetylation between mutants and parentals with p value < 0.05 calculated according to Student’s t test