Skip to main content
Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Histone isoform H2A1H promotes attainment of distinct physiological states by altering chromatin dynamics

Fig. 5

H2A1H-containing nucleosome is more stable than the H2A2A3 nucleosome. a, b Levels of H2A1H and H2A2A3 in the soluble and chromatin fractions upon incubation of CL38 cells in buffers of increasing ionic strength. c FRAP assay performed with CL38 cells expressing YFP-tagged H2A1H and H2A2A3. Recovery was monitored for a period of 1 h. d Graph depicting the percentage recovery of YFP-H2A1H and YFP-H2A2A3 over a span of 4000 s. Error bar represents SEM of ten independent experiments. e Cellular fractionation of the CL38 cells followed by immunoblotting with the marked antibodies to determine the distribution of histones. f RMSD of H2A1H and H2A2A3 nucleosomes over a span of 250 ns of molecular dynamic simulation. g Hydrogen bond analysis of the H2A1H- and H2A2A3-containing nucleosome over the span of 250 ns of molecular dynamic simulation (MDS)

Back to article page

Epigenetics & Chromatin

ISSN: 1756-8935