Fig. 3

Double peptide array experiments confirm bivalent binding of D3PWWP-M8Chromo to H3K9me3 and H3K36me3. a Schematic view of the double peptide arrays. Unmodified, K9me3 and K36me3 modified H3 (1–19) and H3 (26–44) peptides were mixed in all four possible combinations [(1) unmodified H3 (1–19) and unmodified H3 (26-44), (2) H3K9me3 modified H3 (1–19) and unmodified H3 (26–44), (3) unmodified H3 (1–19) and H3K36me3 modified H3 (26–44), and (4) H3K9me3 modified H3 (1–19) and H3K36me3 modified H3 (26–44)] and spotted on a glass slide. See also Additional file 1: Figure S3. b Binding of the D3PWWP-M8Chromo, D3PWWP*-M8Chromo and D3PWWP-M8Chromo* double-HiMIDS to the double peptide array. The array contains two replicates of the four spots. The bar diagrams show the quantification of the double array based on two repetitions. Error bars indicate the SEM