Fig. 9
Asx regulates H3K4me3 and H3K27me3 levels at hsp70. Both panels show ChIP-qPCR analysis comparing control rabbit IgG to trimethylated histones in wild-type (blue) and Asx 3 (red) embryos at different times of heat-shock induction and recovery as indicated in the x-axis. The y-axis indicates recovery after ChIP as a percentage of input DNA. The notation for the duration of heat shock/recovery is described in Fig. 7. All data are represented as mean ± SEM with N = 3. (*) P < 0.05. The standard deviation (SD) of each data point is presented in Additional file 12: Table S3 and Additional file 13: Fig. S6. a ChIP-qPCR analysis with trimethylated histone H3K4 antibody. During late heat-shock induction and the first 30 min of recovery, H3K4me3 levels are higher in Asx mutants than wild type. In late recovery, H3K4me3 levels are lower in mutants than wild type. b ChIP-qPCR analysis with trimethylated histone H3K27 antibody. Levels of H3K27me3 are essentially constant in Asx mutants and are significantly lower than wild type at the end of induction and the recovery phases. c Ratio of histone H3K4me3 to H3K27me3 during heat shock and recovery, expressed in arbitrary units, and derived from the data in a, b