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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Additional sex combs interacts with enhancer of zeste and trithorax and modulates levels of trimethylation on histone H3K4 and H3K27 during transcription of hsp70

Fig. 6

Asx interacts with E(z) and Trx in Drosophila S2 cells in vivo. a Proximity ligation assay (PLA) between Asx and Trx with Drosophila S2 cells before and after heat-shock induction. Nuclei were labeled with DAPI in blue and PLA signals in green for Asx-Trx and in red for Asx-E(z). b PLA between Asx and E(z) with S2 cells before and after heat-shock induction. c, d Schematic diagram of PLA reaction. Oligonucleotides and antibodies used in PLA are listed in “Methods” section. A closed circle between connector and splints forms if proteins are in close proximity. Rolling-circle amplification (RCA) was carried out in the presence of fluorescent-labeled oligonucleotides. Signals would be detected only when proteins were in distance between 10 and 80 nm

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Epigenetics & Chromatin

ISSN: 1756-8935