Fig. 4

Map of Asx summarizing interaction sites of E(z) and Trx SET domains indentified using a GST pull-down assay. a Linear map of Asx showing a bipartite recognition site for both E(z)SET and TrxSET domains. The NH2 terminal recognition site (residues 1–354) termed E(z)-Trx SET domain interaction site 1 (ETSI-1) and the COOH terminal site (residues 1200–1501) termed ETSI-2 are marked above in blue. The single weak recognition site for TrxSET domain (residues 1501–1669), termed TSI, is marked in orange. The ETSI-1 site overlaps both the conserved AsxH domain (residues 200–354) and the Calypso binding site (marked below in green). b Summary of the GST pull-down analysis of the interaction between ³⁵S-labeled Asx deletion fragments with GST fusion proteins of E(z) SET domain or Trx SET domain after a 0.6 M NaCl wash. Non-binding Asx fragments A(1–1200), A(1–200), A(200–354) and A(1381–1501) are indicated by (−). Scouring of the interactions of the Asx deletion fragments with GST-E(z) SET or GST-Trx SET is based on the densitometry of band intensity relative to GST [(++; > tenfold),; +; (twofold–tenfold)]