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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Additional sex combs interacts with enhancer of zeste and trithorax and modulates levels of trimethylation on histone H3K4 and H3K27 during transcription of hsp70

Fig. 3

Asx contains two shared interaction sites for GST-TrxSET and GST-E(z)SET. a SDS–PAGE analysis of expression of 35S-labeled RRL-IVT deletion fragments of Asx (3% of the input for GST pull-down assay in Fig. 2b) and exposed for an hour at −70 °C (lanes 1–9). b Identification of two shared interaction sites for GST-E(z)SET and GST-TrxSET (1–354 and 1200–1500) and one unique, weak site for GST-TrxSET (1501–1669) on Asx. GST pull-down analysis of the Asx fragments in A) with GST-E(z)SET (lane 3) and GST-TrxSET (lane 4) compared to GST controls (lane 1). ()* Estimated fold binding of Asx with GST fusions relative to GST (determined by densitometry) is shown beneath each lane. Unbound fragments, as shown for A(1–1200), are listed in Fig. 3b

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Epigenetics & Chromatin

ISSN: 1756-8935