Fig. 1
From: Optimized reduced representation bisulfite sequencing reveals tissue-specific mCHH islands in maize
Schematic flowchart of maize RRBS. Candidate restriction enzymes are selected by jointly considering the selected range of fragment size, and their enrichment toward ROIs. Enrichment of a specific genomic ROI (e.g., promoter) is measured by comparing the percentage of ROI in the RR genome against that in the reference genome. The ideal enzymes and the range of fragment sizes were decided when the ROIs were clearly enriched