Fig. 1
From: Vitamin C induces specific demethylation of H3K9me2 in mouse embryonic stem cells via Kdm3a/b
Vitamin C leads to a global reduction in H3K9me2 in ES cells. a Percentage of peptide with unmodified K4, K9, K27 or K36 residues on histone H3 from histone mass spectrometry performed on biological triplicates of untreated and vitamin C-treated ES cells. The percentage of unmodified H3K9 is significantly increased with vitamin C treatment. Asterisk represents P < 0.05 by t test. b Pie charts displaying the prevalence of various PTMs on histone H3 K4, K9, K27 and K36 residues in untreated (blue) and vitamin C-treated (red) ES cells. c Log2 ratio of indicated H3 PTMs in vitamin C-treated versus untreated ES cells. H3K9me2 shows the largest fold change and is decreased following vitamin C treatment. Unmodified H3K9 shows the largest increase. d Western blot for H3K9 PTMs in untreated and vitamin C-treated ES cells. e Immunofluorescence for H3K9 PTMs in untreated and vitamin C-treated ES cells. Scale bar represents 20 μm