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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Drosophila CP190- and dCTCF-mediated enhancer blocking is augmented by SUMOylation

Fig. 5

CP190 speckle number is reduced upon FLAG-dSUMO expression. Constitutively FLAG-dSUMO expressing cells were treated with dsRNA against Smt3 or against Aos plus Uba2 (Aos/Uba2). In both cases, cells were stained with antibodies against FLAG (green) and CP190 (red). Cells were grouped into cells with and without a detectable FLAG signal and analyzed for FLAG signal intensity and the number of CP190 intensity maxima using Fiji, a program for image analysis by ImageJ (Additional file 4: Table S2, Additional file 5: Table S3). a Examples of confocal micrographs. b Correlated FLAG-dSUMO signal after the indicated knockdown shows specific reduction of FLAG-dSUMO. Differences in corrected FLAG-dSUMO signal between mock knockdown and specific knockdown were analyzed by two-tailed Mann–Whitney U test. Significance level is defined as two-tailed asymptotic significance p < 0.05. c CP190-speckle number is significantly decreased in cells with high FLAG-dSUMO amount. Differences in number of CP190 intensity maxima between FLAG-dSUMO-positive and negative cells were analyzed by two-tailed Mann–Whitney U test. Significance level is defined as two-tailed asymptotic significance p < 0.05. Statistics were performed in SPSS® (IBM® SPSS® Statistics 22)

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