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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: Drosophila CP190- and dCTCF-mediated enhancer blocking is augmented by SUMOylation

Fig. 4

Binding of CP190 is increased after FLAG-dSUMO expression. a Binding of CP190 (red), dCTCF (green) and FLAG-dSUMO (yellow) before and after induction of FLAG-dSUMO expression (pale and dark colors) after ChIP-qPCR at dCTCF/CP190 example sites, in % of input. IgG was used as negative control (blue). Sites without any binding of dCTCF and CP190 (Hml neg. and Ubx neg.) were used as control sites. b Binding after ChIP-qPCR at CP190 sites devoid of dCTCF binding. Color code as in (a), cg12054 neg., Su(var)2–10 neg. and lwr neg. were used as negative controls devoid of dCTCF and CP190. c Binding of histone H3 after ChIP-qPCR before and after induction of FLAG-dSUMO expression (pale and dark colors) at dCTCF/CP190 (gray) sites, at CP190 (blue) sites and at negative sites (purple). Statistical analysis with two-tailed t test for significant (p value <0.05) changes of binding

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