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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Asymmetric DNA methylation of CpG dyads is a feature of secondary DMRs associated with the Dlk1/Gtl2 imprinting cluster in mouse

Fig. 2

DNA methylation in the 5′ portion of the Gtl2-DMR displays a high level of hemimethylation. Bisulfite mutagenesis and sequencing of F1 hybrid DNA derived from 7.5 d.p.c. B6 × CAST embryos, 14.5 d.p.c. B6 × CAST12 embryos, 5 d.p.p. B6 × CAST12 liver and adult B6 × CAST12 liver. Individual circles in each row represent one of the 11 potentially methylated CpG dinucleotides analyzed, and each paired row of circles represents the complementary strands of an individual subclone; semicircles to the right indicate the location of the linker connecting the complementary strands. Filled circles represent methylated cytosines, open circles represent unmethylated cytosines, and absent circles represent ambiguous data. Labels to the left identify the PCR subclone analyzed; letters represent independent amplification reactions, while numbers represent individual subclones. Subclones derived from the same amplification that have identical sequence and methylation patterns are grouped together, as it was not possible to determine whether these amplicons were derived from the same or different template molecules

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