Fig. 1

Schematic representing sequences analyzed within the Dlk1/Gtl2 imprinting cluster. a Dlk1/Gtl2 imprinting cluster on mouse chromosome 12, including transcriptional start sites (arrows), transcription units (gray boxes) and differentially methylated regions (black boxes). b Regions of the Dlk1-, IG- and Gtl2-DMRs analyzed by bisulfite mutagenesis and DNA sequencing. Information regarding coding strand CpGs, which were analyzed in previous studies [18, 28] provides context for the current analyses. The regions in which complementary CpG dyads were analyzed at the Dlk1-, IG- and Gtl2-DMRs are 156 bp, 126 bp and 520 bp, respectively, and correspond to positions 109,459,709-109,459,865, 109,528,345-109,528,471 and 109,541,256-109,541,776 (GenBank Accession Number NC_000078.6). Sequence polymorphisms used to distinguish the parental alleles (+); genomic coordinates are listed in the Methods. c MspI/HpaII sites analyzed for 5-methylcytosine and 5-hydroxymethylcytosine (*); genomic coordinates are listed in “Methods”