TY - JOUR AU - Guntrum, Megan AU - Vlasova, Ekaterina AU - Davis, Tamara L. PY - 2017 DA - 2017/06/21 TI - Asymmetric DNA methylation of CpG dyads is a feature of secondary DMRs associated with the Dlk1/Gtl2 imprinting cluster in mouse JO - Epigenetics & Chromatin SP - 31 VL - 10 IS - 1 AB - Differential DNA methylation plays a critical role in the regulation of imprinted genes. The differentially methylated state of the imprinting control region is inherited via the gametes at fertilization, and is stably maintained in somatic cells throughout development, influencing the expression of genes across the imprinting cluster. In contrast, DNA methylation patterns are more labile at secondary differentially methylated regions which are established at imprinted loci during post-implantation development. To investigate the nature of these more variably methylated secondary differentially methylated regions, we adopted a hairpin linker bisulfite mutagenesis approach to examine CpG dyad methylation at differentially methylated regions associated with the murine Dlk1/Gtl2 imprinting cluster on both complementary strands. SN - 1756-8935 UR - https://doi.org/10.1186/s13072-017-0138-0 DO - 10.1186/s13072-017-0138-0 ID - Guntrum2017 ER -