Fig. 8
From: Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation
Correspondence of CDK9 and pS187-H1.4 levels at selected genes. a HeLa cells were treated with DMSO (solvent control) or 500 nM actinomycin D for 1 h. The levels of CDK9 and pS187-H1.4 at the promoters and gene bodies of housekeeping genes were assessed by ChIP-qPCR. b The levels of CDK9 and pS187-H1.4 at the transcription start sites of pluripotency factor genes and housekeeping genes in NT2 cells before and after 7 days of RA treatment were assessed by ChIP-qPCR. Negative control ChIP assays employed non-immune rabbit immunoglobulin (rIg) in place of primary antisera. The data are expressed as percent relative to input DNA (mean ± s.e.m., *p < 0.05; **p < 0.01; ***p < 0.001)