Skip to main content
Fig. 7 | Epigenetics & Chromatin

Fig. 7

From: Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation

Fig. 7

Co-enrichment of CDK9 and pS187-H1.4 at selected genes. a re-ChIP assays were performed on asynchronous HeLa cells using CDK9 antibody in the first ChIP followed by pS187-H1.4 antiserum in the second ChIP or vice versa. The co-enrichment of CDK9 and pS187-H1.4 at selected promoters and bodies of housekeeping genes was assessed by qPCR. b re-ChIP assays were performed on pluripotent NT2 cells as in (a). The co-enrichment of CDK9 and pS187-H1.4 at the promoters of pluripotency genes and housekeeping genes was assessed by qPCR. Negative control re-ChIP assays employed non-immune rabbit IgG (rIg) in place of primary antisera for the first ChIP. The data are expressed as percent relative to input DNA (mean ± s.e.m., *p < 0.05; **p < 0.01; ***p < 0.001)

Back to article page