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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation

Fig. 5

siRNA depletion of CDK9 reduced the global and gene-specific levels of pS187-H1.4. a HeLa cells were transfected with siRNA against CDK9 or control siRNA against luciferase for 72 h. Immunoblotting of whole-cell lysates with the indicated antisera was used to assess CDK9 expression and the abundance and phosphorylation of selected H1 variants. The numbers below each panel indicate densitometry for the CDK9-depleted sample relative to the luciferase siRNA control sample. b HeLa cells were transfected with siRNAs against luciferase or CDK9 and the levels of CDK9 and pS187-H1.4 at the promoters or gene bodies of housekeeping genes were assessed by ChIP-qPCR. Negative control ChIP assays employed non-immune rabbit immunoglobulin (rIg) in place of primary antisera. The data are expressed as the percent relative to input DNA (mean ± s.e.m., *p < 0.05; **p < 0.01)

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