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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation

Fig. 2

Changes in the levels of phosphorylated H1 variants at pluripotency factor gene promoters correlate with their reduced expression in differentiated NT2 cells. a The levels of pS187-H1.4, pS173-H1.2/5 and pS18-H1.5 at the transcription start sites of pluripotency factor genes in NT2 cells before and after 7 days of RA treatment were assessed by ChIP-qPCR. b The levels of pS187-H1.4, pS173-H1.2/5 and pS18-H1.5 at the transcription start sites of housekeeping genes in NT2 cells before and after 7 days of RA treatment were assessed by ChIP-qPCR. Negative control ChIP assays employed non-immune rabbit immunoglobulin (rIg) in place of primary antisera. Custom antisera were used for pS187-H1.4 and pS173-H1.2/5 ChIP. Commercial antisera (Active Motif) was used for pS18-H1.5 ChIP. The data are expressed as the percent relative to input DNA (mean ± s.e.m., *p < 0.05; **p < 0.01)

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