Fig. 2From: Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiationChanges in the levels of phosphorylated H1 variants at pluripotency factor gene promoters correlate with their reduced expression in differentiated NT2 cells. a The levels of pS187-H1.4, pS173-H1.2/5 and pS18-H1.5 at the transcription start sites of pluripotency factor genes in NT2 cells before and after 7 days of RA treatment were assessed by ChIP-qPCR. b The levels of pS187-H1.4, pS173-H1.2/5 and pS18-H1.5 at the transcription start sites of housekeeping genes in NT2 cells before and after 7 days of RA treatment were assessed by ChIP-qPCR. Negative control ChIP assays employed non-immune rabbit immunoglobulin (rIg) in place of primary antisera. Custom antisera were used for pS187-H1.4 and pS173-H1.2/5 ChIP. Commercial antisera (Active Motif) was used for pS18-H1.5 ChIP. The data are expressed as the percent relative to input DNA (mean ± s.e.m., *p < 0.05; **p < 0.01)Back to article page