Fig. 1

H1 variant phosphorylation is altered at specific sites after pluripotent cells differentiate. a Retinoic acid-induced differentiation of NT2 cells was assessed by immunoblotting whole-cell lysates with antibodies specific for the indicated pluripotency factors. b Differentiation of mouse embryonic stem cells after withdrawal of leukemia inhibitory factor (LIF) was assessed by immunoblotting whole-cell lysates with antibodies specific for the indicated pluripotency factors. c H1 variant expression and the global levels of their phosphorylation at specific sites in NT2 and mouse ES cells before (day 0) and after differentiation (day 7 of RA treatment or LIF withdrawal) were assayed by immunoblotting whole-cell lysates with the indicated antisera. Signals for histone H3 demonstrate equivalent loading for the NT2 and mESC samples, respectively. The numbers below each panel indicate densitometry for the day 7 sample relative to the day 0 sample