Fig. 4

Imprinting of Ube3a is developmentally regulated. a Immunofluorescence images of paternal Ube3a^YFP protein expression in the subventricular zone, rostral migratory stream, and olfactory bulb of Ube3a ^m+/pYFP mice. Immature and mature neurons were identified by DCX and RBFOX3 staining, respectively (×10 magnification; scale bar = 100 µm). b Immunofluorescence images of paternal Ube3a^YFP protein expression in the dentate gyrus of Ube3a ^m+/pYFP mice (×10 magnification; scale bar = 20 µm). c Immunofluorescence images of paternal Ube3a^YFP in putative neural stem cells (GFAP labeled radial glia), neural progenitor cells (PSA-NCAM), immature neurons (DCX), and mature neurons (RBFOX3) of the subgranular zone (×43 magnification; scale bar = 20 µm). Abbreviations OB, olfactory bulb; RMS, rostral migratory stream; SVZ, subventricular zone; LV, lateral ventricle; DG, dentate gyrus; GCL, granular cell layer; SGZ, subgranular zone; TOPRO-3, nuclear stain; NSC, neural stem cell