Fig. 2

Maternal Ube3a compensates for loss of paternal Ube3a expression during neurogenesis. a Immunofluorescence images of primary neurospheres derived from the hippocampal formation of prenatal Ube3a ^m+/pYFP and Ube3a ^mYFP/p+ mice. Scale bar = 100 µm. b Western blot analysis of Ube3a and Ube3a^YFP protein in NSC derived from Ube3a ^m+/pYFP, Ube3a ^mYFP/p+ and wild-type mice. c Immunofluorescence images of Ube3a ^m+/pYFP and Ube3a ^mYFP/p+ NSC-derived neurons at 1 and 16 days post-differentiation (DPD; scale bar = 25 µm). d Ube3a^YFP intensity values of paternal Ube3a^YFP and maternal Ube3a^YFP protein levels in primary neurons at 1 DPD (Ube3a ^m+/pYFP: n = 13; Ube3a ^mYFP/p+ n = 14), 4 DPD (Ube3a ^m+/pYFP: n = 14; Ube3a ^mYFP/p+: n = 13), 8 DPD (Ube3a ^m+/pYFP: n = 15; Ube3a ^mYFP/p+: n = 15), and 16 DPD (Ube3a ^m+/pYFP: n = 14; Ube3a ^mYFP/p+: n = 13). Ratios normalized relative to total Ube3a^YFP protein levels at 1 DPD. e Total Ube3a^YFP protein levels in neurons during neuronal development. f Normalized paternal Ube3a^YFP and maternal Ube3a^YFP protein levels in Ube3a ^m+/pYFP and Ube3a ^mYFP/p+ NSC-derived astrocytes at 16 DPD (Ube3a ^m+/pYFP: n = 15; Ube3a ^mYFP/p+: n = 14). Abbreviations TOPRO-3, nuclear stain; n.s., not significant; *p < 0.05; **p < 0.001. Individual data points provided with 95% confidence intervals